Iran's supreme leader Ayatollah Ali Khamenei vowed on Saturday to respond to attacks by Israel and its ally the United States against Tehran and groups it supports in the region.

"The enemies, both the USA and the Zionist regime, should know that they will definitely receive a tooth-breaking response to what they are doing against Iran, the Iranian nation, and the resistance front," said Khamenei at a student gathering in Tehran, referring to the alliance of Tehran-backed groups that include Yemen's Houthis, Lebanese Hezbollah and Palestinian Hamas.

It comes as Washington announced the deployment of additional military assets to the Middle East, including ballistic missile defense destroyers and long-range B-52 bomber aircraft, serving as a warning to Iran as it and Israel trade tit-for-tat strikes.

Meanwhile, Hezbollah said it had launched rockets at an Israeli intelligence base near Tel Aviv in the early hours of Saturday.

Hezbollah and Israel exchanged cross-border fire for nearly a year after Hamas's October 7, 2023 attack on Israel triggered the Gaza war, before Israel ramped up the conflict on September 23.

The group frequently claims to have fired rockets at Israeli bases or urban areas in Israeli territory, and has several times claimed the targeting of Glilot.

The Israeli military said on Saturday that sirens were activated following the arrival of "suspicious aerial targets" from Lebanon and the targets remained under surveillance in an ongoing incident. (AFP)